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    • CommentAuthorjuruble
    • CommentTimeJun 14th 2007 edited
     

    This solution is used to remove the jelly coat from fertilized Xenopus laevis eggs, allowing you to separate out the good eggs.

    Cysteine Solution
    (makes enough to cysteine 1 petri dish of fertilized eggs)

    0.6 g Cysteine
    1.0 g Tris base
    30 ml 20% Steinberg’s solution

    Make fresh each use.

  1.  
    For 4 dishes, and with our current (2009) basic-leaning water:

    1.8g Cysteine
    1.7g Tris base
    90 ml 20% Steinberg's solution

    ***Resulting pH: ~7.52
    • CommentAuthormoshannon
    • CommentTimeMay 27th 2015
     
    Summer 2015:

    1.8 g Cysteine
    1.8 g Tris base
    90 mL 20% Steinberg's solution

    *Resulting pH: ~7.5