Not signed in (Sign In)

Welcome, Guest

You have reached the Lom Lab Protocols Resource, where lab protocols are stored, annotated, and discussed.

Please note that you must apply for an account and/or sign in before you can post a new protocol or comment on an existing one. You do not need to sign in to browse the protocols, however.

If you are interested in posting a new protocol, please be sure to read the Before You Post notice to ensure that our protocols remain standard. Thank you, and enjoy your visit!

 
Lom Lab Home

Vanilla 1.1.2 is a product of Lussumo. More Information: Documentation, Community Support.

    • CommentAuthorpabates
    • CommentTimeNov 17th 2010
     
    1. Save “slide” image as .jpeg
    2. Take picture of micronmeter/ruler at same objective (may use different focus). Save as .jpeg as well.
    3. Click File-->Open and select your images to open both images in Adobe Photoshop
    **Save a copy of both images as raw images in .jpeg format so that you have 2 copies
    4. Click on “slide” image you wish to put scale bar on, so that it is selected and visible in layers palette on right side of the screen (the layer should be titled “Background”).
    5. Create a new layer by clicking Layer-->New-->Layer (or Shift+Ctrl+N) and click OK when prompted.
    6. Click on “ruler” image and click Select-->All (or Ctrl+A)
    7. Click edit-->copy (or Ctrl+C) to copy “ruler” image
    8. Click on the new layer you created in your slide image.
    9. Click Edit-->Paste (Ctrl+V) to paste the “ruler” image in new layer (Layer 1)
    10. While still selecting the “ruler” layer (Layer 1), turn the Opacity down to between 30 and 50% (In layers palette enter a value in the Opacity text box or drag the Opacity pop-up slider).
    • You may need to rotate the “ruler” image. Click Edit-->Transform-->Rotate to rotate in small increments so that lines are perfectly vertical.
    11. Create a new layer by clicking Layer-->New-->Layer (or Shift+Ctrl+N) and click OK when prompted.
    12. In new layer (Layer 2) create a straight line that takes up approximately 1/10 of the slide image, using the micronmeter to determine the length.
    • HINT: View-->Zoom In (or Ctrl+) to make line more exact
    • Click the brush tool in drawing toolbox (on left side of screen). Click shift and draw line to make line perfectly straight.
    • To switch between white and black scale bar lines, click on the double headed arrow in the drawing toolbox next to the black and white overlapped squares before drawing your scale bar.
    13. To drag the scale bar to the lower right corner of the “slide” image click the arrow in the drawing toolbox and click and drag the scale bar.
    14. Right click on the “ruler” layer (layer 1) and select delete layer. Click “yes” when prompted.
    15. Click the “T” button in the drawing toolbox and create a text box.
    • Type the length of the scale bar you drew. Remember to leave a space between the numbers and units (ex. 100 µm not 100µm)
    • HINT: typing “m” in the symbol font will give you the “µ”
    • To switch between white and black text, click the double headed arrow in the drawing toolbox next to the black and white overlapped squares before typing.
    16. Click the arrow in the drawing toolbox and click on the text you just created. Drag text to center of scale bar.
    17. Save image with scale bar as .jpeg and .psd

    ALTERNATE (this method is a bit faster)
    1. Save “slide” image as .jpeg
    2. Take picture of micronmeter/ruler at same objective (may use different focus). Save as .jpeg as well.
    3. Click File-->Open and select your images to open both images in Adobe Photoshop
    **Save image as a raw image in .jpeg format so that you have 2 copies
    4. Click on “slide” image you wish to put scale bar on, so that it is selected and visible in layers palette on right side of the screen (the layer should be titled “Background”).
    5. Create a new layer by clicking Layer-->New-->Layer (or Shift+Ctrl+N) and click OK when prompted.
    6. Click on “ruler” image and click Select-->All (or Ctrl+A)
    7. Click Edit-->Copy (or Ctrl+C) to copy “ruler” image
    8. Click on the new layer you created in your slide image.
    9. Click Edit-->Paste (Ctrl+V) to paste the “ruler” image in new layer (Layer 1)
    10. While still selecting the “ruler” layer (Layer 1), turn the Opacity down to between 30 and 50% (In layers palette enter a value in the Opacity text box or drag the Opacity pop-up slider).
    11. Move the opaque “ruler” image so that it is aligned with the right side of the “slide” image, enabling a scale bar to be drawn in the lower right corner.
    • You may need to rotate the “ruler” image. Click Edit-->Transform-->Rotate to rotate in small increments so that lines are perfectly vertical.
    12. Click on the “slide” layer (“Background”) and draw a straight line takes up approximately 1/10 of the slide image, using the opaque micronmeter to determine the length.
    • HINT: View-->Zoom In (or Ctrl+) to make line more exact
    • Click the brush tool in drawing toolbox (on left side of screen). Click shift and draw line to make line perfectly straight.
    • To switch between white and black scale bar lines, click on the double headed arrow in the drawing toolbox next to the black and white overlapped squares before drawing your scale bar.
    • NOTE: once you draw this line it may not be moved, so be sure the “ruler” image is aligned so that the scale bar will be placed correctly
    13. Right click on the “ruler” layer (layer 1) and select delete layer. Click “yes” when prompted.
    14. Click the “T” button in the drawing toolbox and create a text box.
    • Type the length of the scale bar you drew. Remember to leave a space between the numbers and units (ex. 100 µm not 100µm)
    • HINT: typing “m” in the symbol font will give you the “µ”
    • To switch between white and black text, click the double headed arrow in the drawing toolbox next to the black and white overlapped squares before typing.
    15. Click the arrow in the drawing toolbox and click on the text you just created. Drag text to center of scale bar.
    16. Save image with scale bar as .jpeg and .psd