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    • CommentAuthoraldeal
    • CommentTimeJun 12th 2012
     

    Dilute 10X PEI with BBS by factor of 10 (final solution of 0.05% w/v PEI in BBS).


    Keeps in 4 °C refrigerator for about 1-3 months.


    Apply to acid cleaned cover slips as described in protocol “Master resource: Xenopus laevis explant cultures” in the section “Polyornithine treatment