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    • CommentAuthorahrathore12
    • CommentTimeFeb 7th 2015 edited
     
    1) Turn off the brightness for your microscope. The easiest method is simply using the lamp intensity slider and bring it down to P (a green light will still be shown, although it does not impact the imaging).

    2) Turn on the Lumen 2000 (bulb light). Sign in on the paper.

    3) Open the shutter to allow the fluorescence light to reach the sample.

    4) Adjust the Filter Cube Turret (located right below the eyepieces) to a fluorescence of your choosing.
    *Remember: the color emitting from the objective (excitation) will differ from the color actually observed through the eyepieces (emission). That is, when you will see green being excited onto the slide, but when you look through the eyepiece a red fluorescence will be observed.

    5) Turn the objective to the 10x magnification. Try to locate the specimen under the fluorescence. Move onto higher objectives when needed. Not all specimens are fluorescenced in the same way, so you may have to turn the Filter Cube Turret a couple of times. This will take brief trial and error period.

    6) Once you have a well fluorescenced image, go to CellSens and repeat as before with brightfield and phase contrast imaging.
    * Instead of using the White Balance tool, use the Black Balance tool located to the right. This will give a black background which will provide higher contrast for images.

    7) Make sure to sign out once completed.

    *Excessive excitation can potentially be damaging for fluorescence. In other words, don't shine the light onto your specimen for too long, or unnecessarily.