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    • CommentAuthorjuruble
    • CommentTimeJun 15th 2007 edited
     
    Serum Free Culture Media
    (makes 100 ml)

    1 ml pen/strep antibiotic cocktail (AKA Fungibact)
    0.1 g Albumin, bovine serum (fatty acid free, Sigma# A8806)
    39 ml ddH2O
    60 ml L15

    Adjust pH to ~7.4. Sterile Filter prior to using; do not autoclave.
    • CommentAuthorvbeamer
    • CommentTimeMay 14th 2012 edited
     
    The wrong pH may affect the growth of neurites, so make sure the pH meter is calibrated when checking the pH of SFCM.