Not signed in (Sign In)

Welcome, Guest

You have reached the Lom Lab Protocols Resource, where lab protocols are stored, annotated, and discussed.

Please note that you must apply for an account and/or sign in before you can post a new protocol or comment on an existing one. You do not need to sign in to browse the protocols, however.

If you are interested in posting a new protocol, please be sure to read the Before You Post notice to ensure that our protocols remain standard. Thank you, and enjoy your visit!

 
Lom Lab Home

Vanilla 1.1.2 is a product of Lussumo. More Information: Documentation, Community Support.

    • CommentAuthorjuruble
    • CommentTimeJun 15th 2007 edited
     

    AChE stain solution
    (makes ~98 ml)

    AChE Solution 1 (will be blue)
    40 ml 0.1 M acetate buffer
    28 mL ddH2O
    3.2 mL glycine/copper solution
    2 ml 8.8 mM promethazine

    AChE Solution 2 (will be brown/yellow)
    18 ml ddH2O
    372 mg acetylthiocoline iodide
    6 ml 0.1 M copper sulfate

    Both solutions must be made fresh each time. Filter solution 2 with filter paper and then mix with solution 1. Cover tissue/slides in the combined solution and place on rocker/belly dancer for 24-72 hours, then rinse tissue/slides in ddH2O for five minutes. Repeat rinse three times.