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    • CommentAuthorjuruble
    • CommentTimeAug 24th 2007 edited
     

    This protocol explains how to: use the Millipore water systems to obtain either deionized or ultrapure water.
    Protocol revised: 8/24/2007
    Protocol written by: Julie Ruble

     

    NOTE:  While following this protocol, please refer to the pictures of the Millipore water system (below each section).

     

    Obtaining water from Dana 256A and Watson 147A

    You can obtain deionized water or ultrapure water from Dana 256A and Watson 147A.  Use the following guidelines to decide which water you need:

    Deionized water:  Used for making solutions and in "scientific experiments where the presence of impurities may be undesirable"  (from Wikipedia).  This is acceptable for most uses in the lab.

    Ultra-pure water:  Cannot be stored for longer than 1 day, or it is no longer "ultra-pure."  Typically used for sterile procedures, running gels, etc.  From Wikipedia, "The uses of ultrapure deionized water are many and varied, often having applications in scientific experimentation such as when very pure chemical reagent solutions are needed in a chemical reaction or when a biological growth medium needs to be sterile and very pure. Laboratory grade ultra pure water cannot be stored in glass or plastic containers because such materials leach contaminants at very low concentrations into the water. Storage vessels made of silica are used for less demanding applications but for highest purity uses, containers made from ultra pure tin are used."

     

    1. To obtain deionized water, attach tubing to the nozzle on the tank.  You can also forgo the tubing, place carboy/container on counter and fill straight from the tank.  Turn blue lever to start water.  Close lever when carboy/container is full.  You do not have to push any buttons on the Elix (deionizer) to obtain water.  You must stand with your container as it is filling.
    • The upper right corner of the screen on the Elix shows how full the tank is.  Since the Elix starts filtering water when the tank gets down to 75-80% full, the tank should rarely be empty.  The Elix filters 5 liters per hour and the tank holds 60 liters, so if it is emptied, it takes 12 hours to refill.
    • You may press the "measure" button repeatedly to cycle through the temperature, mOhms, etc. of your water, but once again, you do not have to push any buttons to obtain water.
    1.  To obtain ultra-pure water, attach clean tubing to nozzle of the Milli-Q ultra-purifier.  You can also forego the tubing and simply place the nozzle over the container you're filling. Slide trigger back to start water.  Click trigger off when container is full.  You do not have to push any buttons on the Milli-Q ultra-purifier.  DO NOT OPEN THE BLUE DOOR; if you do, the filter canister pops out and water floods the room. You must stand with your container as it is filling.
    • You may press the "measure" button repeatedly to cycle through the temperature, mOhms, etc. of your water, but once again, you do not have to push any buttons to obtain water.

     

    NOTE:  If "service" or "alarm" LEDs are blinking on the Elix or Milli-Q machines, contact Chris Healey for Dana 256A (chhealey@davidson.edu) or Peggy Maiorano for Watson 147A (pemaiorano@davidson.edu).

     

    Elix (deionizer)  Tank Milli-Q purifier

     

    Obtaining deionized or ultra-pure water from Watson 248A

    Use the guidelines above to determine whether you need deionized or ultrapure water.

    1. To obtain deionized water, attach tubing to the nozzle on the tank.  You can also forgo the tubing, place carboy/container on counter and fill straight from the tank.  Turn blue lever to start water.  Close lever when carboy/container is full.  You do not have to push any buttons to obtain water.  You must stand with your container as it is filling.
    1. To obtain ultra-pure water, place container under the nozzle of the ultra-purifier.  This dispenses water purified by UV light and, if a bio-pack is attached to the nozzle, a 0.2 micron filter.  Press dispense button to toggle water on.  Press dispense button again to shut water off.  You do not have to push any other buttons.  DO NOT OPEN THE BLUE DOOR.  You must stand with your container as it is filling.

     

    NOTE: To determine what symbols on the screen mean, use the screen decoder card stored in the bottom slot of the ultra-purifier.  Contact Chris Paradise with any problems (chparadise@davidson.edu).

     

     

    Ultra-pure water  Tank