Not signed in (Sign In)

Welcome, Guest

You have reached the Lom Lab Protocols Resource, where lab protocols are stored, annotated, and discussed.

Please note that you must apply for an account and/or sign in before you can post a new protocol or comment on an existing one. You do not need to sign in to browse the protocols, however.

If you are interested in posting a new protocol, please be sure to read the Before You Post notice to ensure that our protocols remain standard. Thank you, and enjoy your visit!

 
Lom Lab Home

Vanilla 1.1.2 is a product of Lussumo. More Information: Documentation, Community Support.

    • CommentAuthorkalayman
    • CommentTimeSep 14th 2009 edited
     

    Tricaine Solution:

    400 mg tricaine in 97.9 ml E3

    0.26 g Tris in 2.1 ml E3

    Mix Tricaine and Tris in a conical tube and Adjust the pH to~7. Label the tube with the date. Then store the solution in the normal freezer.

     

    Before using as an anesthetic, dilute the concentrate:

    4.2 ml tricaine solution

    ~100 ml E3

     

    This makes the 10x Adult Anesthetic

    • CommentAuthorkaswart
    • CommentTimeOct 6th 2010
     
    Note: this concentration is for the baby zebrafish, not adults.
    • CommentAuthorbrross
    • CommentTimeJun 6th 2011
     
    To use this on baby zebra fish, leave more E3 in the petri dish when anesthetizing.