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    • CommentAuthorkalayman
    • CommentTimeSep 28th 2009
     

     

    Liquid LB

    10 g Tryptone
    5 g Yeast Extract
    10 g NaCl

    Bring to 1 liter with Millipore water
    Adjust pH to 7.5
    Pour into multiple small bottles and autoclave with lid loosened
    (Usually increments of 200-250 mls)