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    • CommentAuthoraldeal
    • CommentTimeJun 18th 2010
     
    This protocol explains how to: remove coverslip from the bottom of a poly-o movie dish

    Protocol revised: 6/18/2010

    Protocol written by: Alex Deal

    This protocol was obtained from the MatTek Corporation website (http://www.glass-bottom-dishes.com/faq.html) under section A-DU5:

    1. Order Part # PDCF OS 30 (Fluid for removal of coverslips from glass bottom dishes)

    2. Invert the cover of the dish.

    3. Pipette 1.0 ml of fluid into the inverted cover.

    4. Place the bottom of the dish in the cover. Make sure that the liquid is touching the bottom of the coverslip.

    5. Allow the dish to sit in the fluid for 45 minutes at room temperature.

    6. Dry the bottom of the coverslip with an absorbent paper towel.

    7. Place the dish on a clean surface. Using forceps, press down on the edge of the coverslip to separate the coverslip from the dish.

    Note: If the above procedure is followed, the PDCF OS 30 fluid will not contact the cells and will not disrupt cells on the coverslip or the staining thereof.