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    • CommentAuthoraadeal
    • CommentTimeNov 17th 2010 edited

    This protocol explains how to: Handle $100 slides

    Protocol revised: 11/17/10

    Protocol written by: Aaron Deal

    1. $100 slide bags should be located in a fix refrigerator

    2. Transport slides without removing from the bags to the microscope of your choice
    3. Once you are ready to view a slide, remove the slide from the plastic container
    4. Like all fluorescent slides, minimize the exposure time of the slide to fluorescent, as well as room, light. Only open the fluorescence filter for short periods or when taking an image
    5. Once the desired image is taken, return the slide to the plastic container
    6. Place the plastic container back into the $100 slide bag
    7. Return bags to fix refrigerator

    Remember: Even though the $100 slides are much more resistant to photobleaching than a usual fluorescent slide, photobleaching is still occurring but at a slower rate. Therefore, the duration of fluorescence on the slides can be extended by reducing exposure to a light source.

    • CommentAuthorpulakhmani
    • CommentTimeDec 15th 2010
    It's not a bad idea to take only one out at a time. If you have multiple hundred dollar slides outside of the plastic container, you may lose one.