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    • CommentAuthorpulakhmani
    • CommentTimeNov 17th 2010 edited
    This protocol explains how to: Refill the portable air tank used for microinjections
    Protocol revised: 11/16/2010
    Protocol written by: Puneet Lakhmani
    1. If attached to picospritzer, use wrench to detach air tank from picospritzer apparatus.
    See Picture:
    Note: wrench should be attached to portable air tank via a carabiner.
    2. Take portable air tank to fume hood where a blue hose connected to an air supply is located.
    3. Connect the hose tip to head of portable air tank (the part not connected to the picospritzer). You have to push a little bit. Note: this whole process will be easier if you rest the air tank on a stationary object e.g. chair while you’re filling.
    See Picture:
    4. At this point, you should hear the tank filling up with air. Wait until the tank is filled to the optimum level. Do not exceed 125 PSI.
    5. Return tank to desired location. Reattach the tank to picospritzer apparatus, making sure the connection is wrench tight.
    • CommentAuthoraadeal
    • CommentTimeDec 14th 2010

    With our air hose (the one in the hood), there is no risk of filling the tank to dangerous levels because it does not have the pressure capable to do so. You can just keep filling until you hear the air stop filling (the hissing stops). That might be around 125 PSI anyway but there is no need to worry about filling the tank too much.