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    • CommentAuthorjuruble
    • CommentTimeJun 22nd 2007
    This protocol explains how to: fire-polish pipettes (can be used in cysteining, in vitro experiments, etc.)

    Protocol revised: 4/16/2007

    Protocol written by: Julie Ruble

    1. Use shorter, cotton-stuffed (if you're using it for sterile cultures) glass pipettes.
    2. Use diamond-tipped scorer in "live dissection tools" drawer (looks like a metal pencil) to score where you want to break the pipette off.  Use the rubber-capped end to tap the tip of the pipette off.  Dispose of the tip in the glass waste box.
    3. Turn on gas and use striker to ignite bunsen burner.
    4. Hold jagged tip of pipette in flame and rotate for ~20-30 seconds or until edges become smooth.  Be careful!  Glass doesn't change its appearance even when very hot.
    5. Turn off gas to extinguish burner.
    • CommentAuthorjoiordanou
    • CommentTimeNov 10th 2007 edited

    When making batches of multiple fire-polished pipettes, score tips and break all the pipettes before you turn on the gas to reduce gas use and chance of getting burned.

    • CommentAuthorkilang
    • CommentTimeNov 11th 2007
    -When tapping the scored tip to break it off, I found it helpful to elevate the pipette just a little bit (by resting it on another scorer) and to tap closer to the tip than the scored line (gave a cleaner break)

    -The longer the tip is in the flame, the smaller the hole gets.
    • CommentAuthorcocron
    • CommentTimeNov 12th 2007
    The pipette tips may take more than a few minutes to completely cool; do not touch the upper half of the pipette until well after they have cooled.