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    • CommentAuthorjuruble
    • CommentTimeJun 22nd 2007 edited

    This protocol explains how to: use the autoclave to sterilize materials.

    Protocol revised: 4/19/2007

    Protocol written by: Julie Ruble

    1. Wrap items such as pipettes in tinfoil before autoclaving. Put materials to be autoclaved in an autoclave bin.
    2. Put a piece of autoclave tape on each object you are autoclaving. Autoclave tape helps you judge when things are sterile by turning "striped" when steam sterilization is complete. Put materials in autoclave.
    3. Turn on "generator control" and close the door all the way. Once door is closed all the way, wait until the "Jacket" pressure reaches at least 20.  This can take about an hour.
    4. Press the "Dry" button and make sure the "Sterilize" and "Dry" settings are correct. We use 20 and 10, respectively, to sterilize our pipettes.
    5. Press "On" button and watch to be sure "Chamber" pressure passes the red tape on the gauge.  You can then leave and come back later to retrieve your sterilized material (time depends on your settings in step 4).
    6. When time remaining is 0 and pressure has returned to 0, you can open the autoclave away from your body.