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    • CommentAuthorkalayman
    • CommentTimeSep 14th 2009 edited

    Tricaine Solution:

    400 mg tricaine in 97.9 ml E3

    0.26 g Tris in 2.1 ml E3

    Mix Tricaine and Tris in a conical tube and Adjust the pH to~7. Label the tube with the date. Then store the solution in the normal freezer.


    Before using as an anesthetic, dilute the concentrate:

    4.2 ml tricaine solution

    ~100 ml E3


    This makes the 10x Adult Anesthetic

    • CommentAuthorkaswart
    • CommentTimeOct 6th 2010
    Note: this concentration is for the baby zebrafish, not adults.
    • CommentAuthorbrross
    • CommentTimeJun 6th 2011
    To use this on baby zebra fish, leave more E3 in the petri dish when anesthetizing.